protSeedCont_M_pct
INRA
Protein %;Content of proteins in seeds determined by NIRS;Pr
Anne Laperche
Rothamsted
SdCampesterolDiunsat_M_mgkg
Richard Broughton, Frederic Beaudoin
SdCampesterol_Cp_pct
Rothamsted
Richard Broughton, Frederic Beaudoin
Sda-toc_M_mgkg
University of York
Swen Langer, Lenka Havlickova
7
regular
Nottingham
LfCu_M_mgkg
Martin Broadley,Thomas Alcock
hailDmg_E_0to9
Hail Damage;HAILDMG
INRA
Anne Laperche
Yongju Huang, John West
University of Hertfordshire
LLSleaf_E_pct
INRA
Growth Stage F1;First flowers are open
corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale);GRWSTG_F1
Anne Laperche
stageF1_cnt_dayj
Nottingham
MLRL_Cp_cm
Martin Broadley, Catherine Thomas
Thomas Alcock, David Salt
Nottingham
SdFe_M_mgkg
Martin Broadley,Thomas Alcock
LfCa_M_mgkg
Nottingham
acronym
1
high > 90%
Thomas Alcock, David Salt
Nottingham
SdCa_M_mgkg
Martin Broadley,Thomas Alcock
LfMg_M_mgkg
Nottingham
Plants with scattered lesions
3
NA
0
Rothamsted
Richard Broughton, Frederic Beaudoin
SdB-Sitosterol_Cp_pct
Sdy-toc_M_mgkg
University of York
Swen Langer, Lenka Havlickova
INRA
climaticAnnot_E_cmt
Climatic Stress;Climatic incidents during the crop cycle;CLIMATIC
Anne Laperche
Anne Laperche
Crop problems;Crop problems during the cycle.;CROP
INRA
cropAnnot_E_cmt
100% girdling ( stem weak/firm)
6
Yongju Huang, John West
University of Hertfordshire
PhomaSKupper_E_0to7
Martin Broadley,Thomas Alcock
LfTi_M_mgkg
Nottingham
SdAlcI_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
SdAs_M_mgkg
Thomas Alcock, David Salt
Nottingham
Seed Moisture Lab;Moisture content in a sample of cleaned seeds determined by weighing;SEEDH2O_LAB
Anne Laperche
seedMoist_mlab_pct
INRA
Thomas Alcock, David Salt
Nottingham
SdNa_M_mgkg
Nottingham
LfS_M_mgkg
Martin Broadley,Thomas Alcock
No leaf spotting
0
0
no symptoms observable
1
trace
Nottingham
LfSe_M_mgkg
Martin Broadley,Thomas Alcock
5
moderately regular
LLSstem_E_pct
Yongju Huang, John West
University of Hertfordshire
Anne Laperche
plntStdEmerg_M_plt
Plant Emergence;Percentage of seeds that emerge;EMERG
INRA
Thomas Alcock, David Salt
Nottingham
SdMn_M_mgkg
entity
Rothamsted
Richard Broughton, Frederic Beaudoin
SdBranched_M_mgkg
Oil Yield;Oil yield per ha;OY
INRA
Anne Laperche
oilYield_Comp_qha
INRA
corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale);GRWSTG_C1
stageC1_cnt_dayj
Growth Stage C1;Bolting date when the first inter-node appears
Anne Laperche
Rachel Wells
PedLen_M_cm
John Innes Centre
Nottingham
SdSr_M_mgkg
Thomas Alcock, David Salt
INRA
Seed Moisture NIRS;Moisture content in a sample of seeds determined by NIRS;SEEDH2O_NIRS
seedMoist_mnirs_pct
Anne Laperche
stageG5_cnt_dayj
INRA
seeds are brown and hard. Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale);GRWSTG_G5
Growth Stage G5;10% of the seeds have reached maturity
Anne Laperche
Thomas Alcock, David Salt
Nottingham
SdCu_M_mgkg
Richard Broughton, Frederic Beaudoin
Rothamsted
SdBrassicasterol_Cp_pct
Anne Laperche
Plant Lodging;Scoring of the plant lodging of the plot;LODGING
lodging_E_0to9
INRA
Plant Density;Number of viable plants counted per surface unit;PLANTDENS
Anne Laperche
plntStnd_M_plntm2
INRA
Nottingham
LfAl_M_mgkg
Martin Broadley,Thomas Alcock
PodNumbTermRac_Ct_PodTermRac
Rachel Wells
John Innes Centre
PodWid_M_cm
Rachel Wells
John Innes Centre
TRL_Cp_cm
Nottingham
Martin Broadley, Catherine Thomas
plotHarvest_M_kg
INRA
Plot Harvest Weight;Weight (kg) of raw materials harvested by plot;PLOTHARVEST
Anne Laperche
Yongju Huang, John West
University of Hertfordshire
white blight
Sclerot_Cou_plnt6plnt
Martin Broadley,Thomas Alcock
LfSr_M_mgkg
Nottingham
Nottingham
LfRb_M_mgkg
Martin Broadley,Thomas Alcock
INRA
palmiticSeedCont_M_pct
Palmitic Acid;Content of palmitic acid in seeds determined by NIRS;PALMITIC
Anne Laperche
60-90%
3
Rachel Wells
John Innes Centre
PltHght_M_cm
INRA
protMealCont_M_pct
Protein Meal %;Content of proteins in the meal determined by NIRS;PRMEAL
Anne Laperche
LfCd_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
INRA
regStand_E_0to9
Anne Laperche
Regularity Stand;Scoring of the regularity of the plant stand;REGSTAND
9
perfectly regular
LLSpods_E_pct
Yongju Huang, John West
University of Hertfordshire
No disease observable
0
5
intermediate damage
Phoma damage G2 score
Anne Laperche
INRA
phomaDmg_CompG2_0to9
INRA
Erucic Acid;Content of erucic acid in seeds determined by NIRS;ERUCIC
erucicSeedCont_M_pct
Anne Laperche
University of Hertfordshire
LLSleaf_E_0to7
Yongju Huang, John West
SdS_M_mgkg
Thomas Alcock, David Salt
Nottingham
Thomas Alcock, David Salt
Nottingham
SdK_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
LfAg_M_mgkg
Thomas Alcock, David Salt
SdCd_M_mgkg
Nottingham
Yongju Huang, John West
University of Hertfordshire
PhomaLS_Cou_plnt10plnt
Martin Broadley, Catherine Thomas
LRN_Ct_root
Nottingham
9
< 10%
SdMg_M_mgkg
Thomas Alcock, David Salt
Nottingham
Martin Broadley,Thomas Alcock
LfMo_M_mgkg
Nottingham
Martin Broadley, Catherine Thomas
LRD_Cp_rootcm
Nottingham
Anne Laperche
corresponds to growth stage 69 (BBCH scale);GRWSTG_ENDFLO
stageEndFlo_cnt_dayj
INRA
Growth Stage End Flowering;End of flowering on the main inflorescence
Anne Laperche
INRA
Nitrogen Harvest Index;Proportion of N accumulated in seed to N accumulated in the total plant (including shoot and root);NHI
NHI_Comp_pct
Growth Stage G1;10% of the pods have reached their final size
stageG1_cnt_dayj
INRA
flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale);GRWSTG_G1
Anne Laperche
Richard Broughton, Frederic Beaudoin
SdStigmasterol_Cp_pct
Rothamsted
Nottingham
LfP_M_mgkg
Martin Broadley,Thomas Alcock
Growth Stage Pod Development;Additional stages for pod development according to the BBCH scale (72-79);GRWSTG_PODDEV
Anne Laperche
stagePodDev_cnt_dayj
INRA
seedMoistHarv_mhumi_pct
Seed Moisture Harvest;Moisture content in a sample of seeds determined by humiditer;SEEDH2O_HARV
INRA
Anne Laperche
Richard Broughton, Frederic Beaudoin
SdAlk_M_mgkg
Rothamsted
Nottingham
LfK_M_mgkg
Martin Broadley,Thomas Alcock
Rothamsted
Richard Broughton, Frederic Beaudoin
SdKeto_M_mgkg
3
>10 spots per plant
Martin Broadley,Thomas Alcock
LfNi_M_mgkg
Nottingham
4
low to intermediate damage
Yongju Huang, John West
University of Hertfordshire
PhomaLS_Cou_pct
Nottingham
LfZn_M_mgkg
Martin Broadley,Thomas Alcock
4
Diseased leaved with a few large lesions of many small leasions, area quite heavily infected
Thomas Alcock, David Salt
SdSe_M_mgkg
Nottingham
Yongju Huang, John West
University of Hertfordshire
VertWilt_Cou_pct
INRA
stearicSeedCont_M_pct
Stearic Acid;Content of stearic acid in seeds determined by NIRS;STEARIC
Anne Laperche
StemWid_M_cm
Rachel Wells
John Innes Centre
Rothamsted
Richard Broughton, Frederic Beaudoin
SdAvenasterol_M_mgkg
high to severe damage
8
Richard Broughton, Frederic Beaudoin
SdTotalWax_M_mgkg
Rothamsted
SdFA_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
Rothamsted
Richard Broughton, Frederic Beaudoin
SdAvenasterol_Cp_pct
Thomas Alcock, David Salt
Nottingham
SdB_M_mgkg
Anne Laperche
validateannot_E_cmt
INRA
Annotations on measure validity;VALIDATION
Seed Impurities;Percentage of impurities in a sample of seeds determined by weighing;SEEDIMPUR
Anne Laperche
seedImpur_M_pct
INRA
flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale);GRWSTG_G4
Growth Stage G4;30% of the pods have reached their final size
Anne Laperche
INRA
stageG4_cnt_dayj
Rachel Wells
John Innes Centre
FertBraNumb_Ct_Fb
SdBrassicasterol_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
oleicSeedCont_M_pct
Anne Laperche
INRA
Oleic Acid;Content of oleic acid in seeds determined by NIRS;OLEIC
Thomas Alcock, David Salt
Nottingham
SdLi_M_mgkg
Yongju Huang, John West
University of Hertfordshire
LeafMiner_Cou_pct
Yongju Huang, John West
Sclerot_Cou_plntPlot
University of Hertfordshire
white blight
Anne Laperche
podShat_E_score
INRA
Pod shattering;Scoring of pods open just before harvest;PODSHAT
INRA
Weevils (all species) damage score;charancon
Anne Laperche
weevilsDmg_E_0to9
Anne Laperche
linoleicSeedCont_M_pct
Linoleic Acid;Content of linoleic acid in seedsdetermined by NIRS;LINOLEIC
INRA
SdMo_M_mgkg
Thomas Alcock, David Salt
Nottingham
Rothamsted
Richard Broughton, Frederic Beaudoin
SdAlcII_M_mgkg
1
<5 spots per plant
Growth Stage Flowering;Additional flowering stages according to the BBCH scale (62-65);GRWSTG_FLO
Anne Laperche
INRA
stageFlo_cnt_dayj
SdWght_M_mg
Rachel Wells
John Innes Centre
Martin Broadley,Thomas Alcock
LfMn_M_mgkg
Nottingham
glsSeedCont_M_μmolg
Anne Laperche
Glucosinolates;Content of glucosinolates in seeds determined by NIRS;GLS
INRA
LfCs_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
VertWilt_E_0or1
University of Hertfordshire
Yongju Huang, John West
SdCycloartenol_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
University of Hertfordshire
PhomaSKbase_E_0to7
Yongju Huang, John West
Rothamsted
Richard Broughton, Frederic Beaudoin
SdB-Sitosterol_M_mgkg
Rothamsted
SdCampesterol_M_mgkg
Richard Broughton, Frederic Beaudoin
7
10-30%
Nottingham
Martin Broadley,Thomas Alcock
LfV_M_mgkg
Sdd-toc_M_mgkg
University of York
Swen Langer, Lenka Havlickova
Anne Laperche
stageF2_cnt_dayj
Growth Stage F2;10% of the flowers are open on the main inflorescence
INRA
elongation of the main inflorescence. Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale);GRWSTG_F2
2
bioticAnnot_an_text
Anne Laperche
Biotic Stress;Biotic attack during the crop cycle;BIOTIC
INRA
LfNa_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
plotSize_M_m2
Anne Laperche
Plot Surface;Surface of the elementary experimental plot;PLOTSURF
INRA
3
26-50% girdling
SdCr_M_mgkg
Thomas Alcock, David Salt
Nottingham
Nottingham
Martin Broadley,Thomas Alcock
LfBa_M_mgkg
2
Culture leaning, without impact
Anne Laperche
plntHeight_M_cm
INRA
Plant Size;Height of plants in an experimental plot;PLANTSIZE
Oil %;Content of oil in seeds determined by NIRS;O
INRA
oilSeedCont_M_pct
Anne Laperche
FtLen_M_cm
Rachel Wells
John Innes Centre
Rachel Wells
John Innes Centre
PodLen_M_cm
SdCycloartenol_Cp_pct
Richard Broughton, Frederic Beaudoin
Rothamsted
INRA
Growth Stage Pod Maturity;Additional stages for pod maturity according to the BBCH scale (82-99);GRWSTG_PODMATURITY
stagePodMaturity_cnt_dayj
Anne Laperche
3
Brassica napus ontology
Brassica ontology
Nottingham
SNi_M_mgkg
Thomas Alcock, David Salt
SdAld_M_mgkg
Rothamsted
Richard Broughton, Frederic Beaudoin
Martin Broadley,Thomas Alcock
Nottingham
LfFe_M_mgkg
University of Hertfordshire
PowMildew_E_0or1
Yongju Huang, John West
6
Thomas Alcock, David Salt
Nottingham
SdP_M_mgkg
Linolenic Acid;Content of linolenic acid in seeds determined by NIRS;LINOLENIC
Anne Laperche
linolenicSeedCont_M_pct
INRA
5-10 spots per plant
2
SdRb_M_mgkg
Thomas Alcock, David Salt
Nottingham
Richard Broughton, Frederic Beaudoin
SdStigmasterol_M_mgkg
Rothamsted
Rachel Wells
BeakLen_M_cm
John Innes Centre
Martin Broadley,Thomas Alcock
LfAs_M_mgkg
Nottingham
Diseased leaves with non or a few small lesions
2
high irregular
1
51-75% girdling
4
Thomas Alcock, David Salt
SdPb_M_mgkg
Nottingham
≤ 5% girdling
1
Yongju Huang, John West
University of Hertfordshire
PhomaSK_E_0to7
Nottingham
Martin Broadley, Catherine Thomas
LRL_M_cm
2-25% girdling
2
protYield_Comp_qha
Anne Laperche
INRA
Prot Yield;Protein yield per ha;PrY
PRL_M_cm
Martin Broadley, Catherine Thomas
Nottingham
No lodging
1
LfPb_M_mgkg
Nottingham
Martin Broadley,Thomas Alcock
SdNum_Ct_sd
Rachel Wells
John Innes Centre
PhomaLS_E_0to3
Yongju Huang, John West
University of Hertfordshire
5
Average loding
75-100% girdling
5
Evidence of stunting
6
1
Trace of disease
irregular
3
Rothamsted
SdPhytosterol_M_mgkg
Richard Broughton, Frederic Beaudoin
intermediate to high damage
6
Nottingham
Martin Broadley,Thomas Alcock
LfCr_M_mgkg
NA
0
Martin Broadley, Catherine Thomas
SD_Est_mm
Nottingham
8
low damage
3
Nottingham
LfB_M_mgkg
Martin Broadley,Thomas Alcock
very low damage
2
Nottingham
Martin Broadley,Thomas Alcock
LfU_M_mgkg
Thomas Alcock, David Salt
Nottingham
SdCo_M_mgkg
Rothamsted
SdCampesterol diunsat_Cp_pct
Richard Broughton, Frederic Beaudoin
100% girdling (stem dead/lodged))
7
0
NA
4
SdZn_M_mgkg
Thomas Alcock, David Salt
Nottingham
7
high damage
4
30-60%
5
Plants heavily stunted or dead.
7
9
severe damage
Half green/half diseased, leaf curling and distortion
5
0
no visible affection
attribute
Campesterol
The percentage of Campesterol in the total of Phytosterols in seeds
SdCampesterol_Cp
Seed
Seed Campesterol content
Campesterol proportion of Phytosterols
GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement
* Roughly 80 mg of seed material is weighed into Precellys grinding tubes (ceramic beads, 2 ml capacity). The large seed amount is to reduce inter-seed variation.
* Seeds are ground at 5500 rpm for 25 seconds three times, with a wait time of 30 seconds between each grinding step.
* 1 ml of hexane containing the internal standards cholesteryl-heptadecanocate and cholestanol at a concentration of 0.00002M (20 µM) are added to samples.
* Samples are vortexed, and then incubated at 30°C in a rotary incubator at 200 rpm for 1 hour.
* Samples were then centrifuged at 14000 rpm for 1 minute to sediment seed material. The hexane supernatant was removed using Pasteur pipettes and transferred to 3 ml, glass trident vials.
* A further two hexane extractions were performed (with no internal standards added), with 1 ml of pure hexane added to each sample, incubation at 30°C for 1 hour at 200 rpm, with a prior sample vortex step, centrifugation at 14000 rpm and finally, separation and pooling of the hexane fractions.
* The 3 ml of seed lipid extract was dried down using a Genevac EZ-2 at ? 40°C for 40 minutes.
* 1.5 ml of 1.5 M potassium hydroxide in 95% ethanol was added to the lipid sample (roughly 10 mg), and incubated at 80°C for 1 hour (from 80 mg total seed, 1ml total suspension, 250 ul used).
* The samples are cooled, and 1 ml of a 1% (w/v) NaCl solution is added, followed by 1 ml of hexane.
* Samples are vortexed, centrifuged and the upper hexane phase is removed.
* A further hexane extraction is carried out with 1 ml of hexane, which is pooled with the first.
* Samples are dried in a rotary evaporator or under a stream of nitrogen.
* Free sterols are then silated using 100 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C. (100 ul heptane added, 1 ul injection into GC-FID).
*GC program:
* Initial temp: 200°C, to 300°C at 16°C/min, then to 325°C at 4°C min and held for 4.5 minutes.
* Inlet temp: 325°C, split-less injection.
* Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min.
* FID temp: 325°C
The concentration of Boron in leaves
Boron content
Leaf Boron content
Leaf
LfB
mg/kg
Pod width - Measurement
Measurement of mean pod width (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged
Seed N content to total plant N content ratio
Nitrogen Harvest Index
N content proportion of plant N content
Ratio of N accumulated in seed to N accumulated in the total plant (including shoot and root)
Seed
NHI
Beginning of pod maturity (BBCH 81) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
BBCH growth stage 60;CETIUM growth stage F1
Development stage
First flowers time (BBCH 60)
Plant
First flowers are open, corresponds to growth stage F1 (CETIOM) or 60 (BBCH scale)
Protein seed content NIRS - Measurement
NIRS assay. Pct of Seed Dry Weight
Pod Number - Counting
Count number of pods in a 20 cm length of the terminal raceme (primary if terminal damaged). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing.
Leaf
Leaf Arsenic content
LfAs
The concentration of Arsenic in leaves
Arsenic content
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
End of flowering (BBCH 69) date – Estimation
Pod and stem
SdTotalWax
The concentration of wax in the pod and seed material
Pod and stem wax content
wax content
Seed Lead content
Seed
Lead content
The concentration of Lead in seeds
SdPb
Oil seed content NIRS - Measurement
NIRS assay. Pct of Seed Dry Weight
B-Sitosterol
Seed
The percentage of B-Sitosterol in the total of Phytosterols in seeds
SdB-Sitosterol_Cp
Seed B-Sitosterol content
B-Sitosterol proportion of Phytosterols
Seed
Stearic acid content
SdStearic
Seed stearic acid content
The concentration of stearic acid in seeds.
Plant
Lodging incidence
Plant lodging incidence
The plant lodging incidence.
Lodg
Seed protein Yield - Computation
Seed protein content is calulated as a product of seed yield (SY) and protein content (expressed as a fraction) in seeds (Pr)
Seed mineral analysis - Measurement
3 seeds per tube and occasionally four for verry small seeds from multiple replicate plants per accession were left to soak in nitric acid over night and then digested in block heaters prior to analysis by inductively coupled plasma-mass spectrometry (ICP-MS). This data was then weight normalised so that data from each element was in the units mg/kg
The concentration of Magnesium in seeds
Magnesium content
Seed
Seed Magnesium content
SdMg
SdCampesterolDiunsat_Cp
Campesterol diunsat
Campesterol diunsat proportion of Phytosterols
The percentage of diunsaturated Campesterol in the total of Phytosterols in seeds
Seed
Seed diunsaturated Campesterol content
Uranium content
The concentration of Uranium in leaves
Leaf Uranium content
LfU
Leaf
Total root
length
Length of all roots
TRL
Total root length
Iron content
Seed
SdFe
Seed Iron content
The concentration of Iron in seeds
SdPalmitic
Seed
Palmitic acid content
Seed palmitic acid content
The concentration of palmitic acid in seeds.
LfV
Leaf
The concentration of Vanadium in leaves
Vanadium content
Leaf Vanadium content
Hail damage
Plant damage caused by hail.
Hail damage
HailDmg
Plant
Pedicel length
pedicel
length
Pedicel length of pods from terminal raceme
B-Sitosterol content
Seed
The concentration of B-Sitosterol in seeds
B-Sitosterol
Seed B-Sitosterol content
SdB-Sitosterol
Beginning of flowering time (BBCH 61)
Development stage
10% of the flowers are open on the main inflorescence, elongation of the main inflorescence Corresponds to growth stage F2 (CETIOM) or 61 (BBCH scale)
Plant
BBCH growth stage 61;CETIUM growth stage F2
Verticilium wilt presence - Counting
Six plants were sampled from each plot. Each whole plant was assessed for presence or absence of verticilium wilt. The % of plants with Verticillium symptoms out of the 6 sampled plants was recorded.
Shattering
Proportion of open pods (assessment)
Pod
Pod shattering
PodShat
Chromium content
LfCr
Leaf Chromium content
Leaf
The concentration of Chromium in leaves
%
Stand
Plant
Plant stand
PltSd
Plant density
Crop stand
Number of viable plants counted per surface unit
Exposure to crop problems;damage from crop problem
Plant
Crop problems annotation
Crop problems during the crop cycle.
CropPb
Meal
Protein content
Meal protein content
MlProt
The concentration of proteins in the meal.
Linoleic seed content NIRS - Measurement
NIRS assay. Pct total fatty acids
Sclerotinia incidence
Plant
No of plants with Sclerotinia (out of six)
Numper of plants that are infected by Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering.
Biotic attack annotation
Biotic attack during the crop cycle.
BioticPb
Exposure to biotic attack; damage du to biotic attack
Plant
* 3 pods and 3 sections of stem (10 cm) were collected from each plant, and inserted into Pyrex glass tubes (122 mm (L) x 19 mm (D), roughly 20 ml vol.).
* Wax was extracted using chloroform. Chloroform was spiked with a C22:0 alkane and C17:0 fatty acid as internal standards. Internal standard concentrations were 10 µM in chloroform (giving 100 pmol/µl for GC injection). Wax was extracted for 30 seconds using 10 ml of the chloroform/IS mixture. Sample tubes were inverted continuously during the extraction phase. PTFE cap liners were used to ensure minimal extraction of plasticisers.
* Pod and stem material are removed from the tubes, and samples dried under vacuum in a centrifugal evaporator (Genevac, EZ-2) at ? 42°C using program low BP. Samples can also be dried under a stream of nitrogen at ? 30°C.
* Wax components were derivatized with 200 µl of BSTFA + TMCS (99:1) for 1 hour at 85°C.
* After cooling, 800 µl of heptane was added (total vol. 1 ml), and 1 µl was injected onto the GC-FID/MS.
* GC program:
* Initial temp: 50°C, to 200°C at 50°C/min, to 280°C at 2°C min, to 325°C at 10°C/min, hold for 6.5 minutes.
* Inlet temp: 280°C, split-less injection.
* Column: HP-1MS UI, 30 m x 0.25 mm x 0.25 µm, flow = 1 ml/ min.
* FID temp: 325°C
GC FID (Gas Chromatography with Flame Ionization Detector) - Measurement
Absence or presence of powdery mildew
Powdery mildew presence
Plant
Presence (1) or absence (0) of powdery mildew
End of flowering time (BBCH 69)
BBCH growth stage 69;end flowering stage
End of flowering on the main inflorescence, corresponds to growth stage 69 (BBCH scale)
Development stage
Plant
The concentration of Boron in seeds
SdB
Seed Boron content
Seed
Boron content
Samples from HI assay are analysed for N_%DW in the different fractions
Nitrate Harvest index - Computation
NIRS assay
Seed moisture NIRS - Measurement
LLS incidence (diseased) - Estimation
10 plants from each plot were assessed on % incidence with light leaf spot (LLS). The incidence of LLS on leaves was recorded based on presence/absence of LLS. For example, 4 out 10 plants with phoma, the incidence of phoma will be 40%.
Beginning of flowering (BBCH 61) date - Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
diameter
SD
Seed diameter
The diameter of a seed
Seed
The percentual proportions of individual Sterols derived from MO_008 were calculated from the concentrations with regard to the total amount of sterols
Relative seed Phytosterol content - Computation
cm
Seed
Campesterol
Seed Campesterol content
The concentration of Campesterol in seeds
SdCampesterol
Campesterol content
NIRS assay. Pct total fatty acids
Linolenic seed content NIRS - Measurement
Leaf
Leaf Zinc content
LfZn
The concentration of Zinc in leaves
Zinc content
Plot measure validity
Plot
Valid
Assessment on measure validity.
Measure validity
Glucosinolate content
The concentration of glucosinolates in seeds.
Seed glucosinolate content
Seed
SdGLS
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Addictional flowering stages (BBCH 62-65) date – Estimation
Erucid seed content NIRS - Measurement
NIRS assay. Pct total fatty acids
Severity assessment of phoma leaf spot
Phoma leaf spot severity
Leaf phoma resistance
Leaf
MLRL
The mean length of a lateral root of that root system
Mean lateral root length
average length
Lateral root
plants/10 plants
pod/20cm of terminal raceme
StOleic
Seed
Seed oleic acid content
Oleic acid content
The concentration of oleic acid in seeds.
SdStigmasterol_Cp
Seed
The percentage of Stigmasterol in the total of Phytosterols in seeds
Stigmasterol proportion of Phytosterols
Stigmasterol
Seed Sigmasterol content
day-of-year
Leaf mineral analysis - Measurement
3-5 leaves per plant were pooled and freeze-dried. These were then ground and digested in nitric acid in a microwave digester prior to analysis by inductively coupled plasma-mass spectrometry
Biotic stress annotations - Estimation
Visual observations
Sdy-toc
gamma-tocopherol content
Seed gamma- tocopherol content
seed
Gamma- tocopherol conten in seeds
SdLi
The concentration of Lithium in seeds
Seed
Seed Lithium content
Lithium content
Leaf phoma resistance - Estimation
10 spots per plant]]>
Sdd-toc
Seed delta- tocopherol content
delta-tocopherol content
seed
Delta- tocopherol content in seeds
First inter-node time (BBCH 31)
Plant
Bolting date when the first inter-node appears, corresponds to growth stage C1 (CETIOM) or 31 (BBCH scale)
BBCH growth stage 31;CETIUM growth stage C1
Development stage
Leaf
The concentration of Molybdenum in leaves
Molybdenum content
LfMo
Leaf Molybdenum content
Seed
Phytosterol content
Seed total Phytosterol content
The total concentration of phytosterols in seeds
SdPhytosterol
Seed
SdCd
The concentration of Cadmium in seeds
Cadmium content
Seed Cadmium content
Comment
The concentration of diunsaturated Campesterol in seeds
Seed
diunsaturated Campesterol content
SdCampesterolDiunsat
Seed diunsaturated Campesterol content
Campesterol diunsat
LfP
The concentration of Phosphorus in leaves
Phosphorus content
Leaf Phosphorus content
Leaf
Climatic incidents annotation
Exposure to climatic stress
ClimPb
Plant
Climatic incidents during the crop cycle.
Early pod development time (BBCH 73)
30% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G4 (CETIOM) or 73 (BBCH scale)
BBCH growth stage 73;CETIUM growth stage G4
Plant
Development stage
NIRS assay. Pct of Seed Dry Weight
Protein meal content NIRS - Measurement
The concentration of components with primary alcohol functional groups from pod and stem material
primary Alkohol functional group content
SdAlcI
Pod and stemprimary Alkohol functional group content
Pod and stem
Beak length
beak
Pod beak length
length
The concentration of Strontium in leaves
Leaf Strontium content
LfSr
Leaf
Strontium content
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Additionnal pod maturity stages (BBCH 82-99) date – Estimation
The concentration of Copper in leaves
Leaf
LfCu
Leaf Copper content
Copper content
10 plants were sampled from each plot. Each plant was assessed for light leaf spot (LLS) on the whole stem (from botton to the top) and the % diseased area estimated.
LLS severity - Estimation
Height is measured from ground to the top of the plants using a dipstick graduated in cm
Plant height - Measurement
Pod length
length
Pod length of pods from terminal raceme
pod
10 plants from each plot were sampled. Each plant was assessed for presence/absence of phoma leaf spots. The number of plants with phoma leaf spots out of the 10 plants was recorded
Phoma leaf spot incidence (diseased) - Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Beginning of pod development (BBCH 71) date – Estimation
A homogenous mixture of 80 mg of rapeseed seeds from each plant were ground in a bead mill (Tissue Lyser, Qiagen, Manchester, UK)
twice for 2min at 30Hz with 750 µl heptane (Rathburn, Walkerburn, UK) and 150 µl of 0.12mg mL-1 (25.4µM) internal standard, alpha-tocopherol acetate (Sigma-Aldrich, Gillingham, UK)
dissolved in ethanol (Rathburn). Samples were incubated at 4°C for 2h, centrifuged and 20 µl of clear supernatant directly subjected to HPLC analysis.
Tocopherols were identified by comparison of retention times with standards. TTC was counted as the sum of a-,y- and d-tocopherol contents
HPLC (High performance liquid chromatography) analysis - Measurement
The concentration of Manganese in leaves
LfMn
Leaf Manganese content
Manganese content
Leaf
Observe damage caused by Weevils (all species), and rate the damage.
Weevil damage - Estimation
Leaf
Leaf Potassium content
LfK
Potassium content
The concentration of Potassium in leaves
Plant
Scoring of the regularity of the plant stand
Stand
Regularity of plant stand
The concentration of Molybdenum in seeds
Molybdenum content
Seed Molybdenum content
Seed
SdMo
10 plants from each plot were assessed and the number of plants with Phoma leaf spot was recorded. The whole plant was assessed. The incidence of Phoma leaf spot was calculated as % of plants out of ten sampled plants that were found to have Phoma leaf spot.
Phoma incidence (diseased) - Counting
LfAg
Leaf Silver content
Silver content
The concentration of Silver in leaves
Leaf
Leaf Selenium content
Leaf
Selenium content
The concentration of Selenium in leaves
LfSe
Plant
Sclerotinia incidence
Presence or absence of fungal infection of Sclerotinia sclerotiorum, a disease also known as 'white blight' , as it produces cottony white mold, on mainly the aboveground parts, which turn into small black 'sclerotia' for overwintering.
No of plants with Sclerotinia (per plot)
0-9 Damage/Incidence scale
SdNa
Sodium content
Seed
Seed Sodium content
The concentration of Sodium in seeds
SdMoist
Seed
Seed moisture content
Moisture content
Seed moisture content.
LLS severity on pods (percentage of pod area covered by LLS)
Pod
Light leaf spot severity
Light leaf spot severity on pods, judged by the percentage area of the pods covered by LLS.
Stem length - Measurement
Measure length of lower stem from ground level to last formed fertile branch (cm) Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing.
Seed
SdZn
Zinc content
The concentration of Zinc in seeds
Seed Zinc content
Linoleic acid content
Seed linoleic acid content
Seed
The concentration of linoleic acid in seeds.
StLinoleic
PlotSize
Size
Surface of the elementary experimental plot
Plot size
Plot
Leaf
Light leaf spot incidence
LLS incidence on leaf
The percentage of plants that showed presence of light leaf spot incidence on leaves
weight
seed
Seed weight
Seed weight of ten typical pods
The concentration of Cobalt in seeds
SdCo
Cobalt content
Seed Cobalt content
Seed
Alkane-functional group content
Pod and stem Alkane-functional group content
SdAlk
Pod and stem
The concentration of components with alkane functional groups from pod and stem material
Incidence of Phoma leaf spot
The percentage of plants that showed presence of phoma leaf spot from a subsample of a plot
Phoma incidence
Leaf
Measurement of mean pod length (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged
Pod length - Measurement
Verticilium wilt presence
Plant
Absence or presence of Verticilium wilt on the plant
Verticilium wilt presence
WeevilsDmg
Plant
Weevil damage caused by the agent Ceutorhynchus napi (Rape Stem Weevil), Ceutorhynchus pallidactylus (cabbage stem weevil) or Ceutorhynchus assimilis/obstrictus (cabbage seedpod weevil).
Weevils damage
Weevils damage
Pod and stem
SdKeto
Pod and stem Ketone functional group content
Ketone functional group content
The concentration of components with ketone functional groups from pod and stem material
LfCd
Cadmium content
Leaf Cadmium content
Leaf
The concentration of Cadmium in leaves
Lead content
LfPb
Leaf Lead content
Leaf
The concentration of Lead in leaves
0-7 percentage scale
LfBa
Leaf Balium content
The concentration of Balium in leaves
Balium content
Leaf
LfS
Sulphur content
The concentration of Sulphur in leaves
Leaf
Leaf Sulphur content
fertile branch
Leaf
LfMg
Magnesium content
Leaf Magnesium content
The concentration of Magnesium in leaves
root/cm
Strontium content
The concentration of Strontium in seeds
Seed Strontium content
SdSr
Seed
Leaf Titanium content
Leaf
Titanium content
LfTi
The concentration of Titanium in leaves
root
Seed
Phosphorus content
Seed Phosphorus content
The concentration of Phosphorus in seeds
dSP
plant
Plant height
height
Maximum plant height from ground to tip of terminal raceme
PH
A measurement of the amount (in kg) of raw material that was harvested per plot as determined by on-board weighing on the harvester
plotharvest - Measurement
Seed Sulphur content
Sulphur content
Seed
SdS
The concentration of Sulphur in seeds
Brassicasterol
Seed Brassicasterol content
The percentage of Brassicasterol in the total of Phytosterols in seeds
Seed
Brassicasterol proportion of Phytosterols
SdBrassicasterol
Early pod development (BBCH 73) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Image analysis - Computation
Density of lateral roots (LRD) occuring along the primary root were calculated by dividing lateral root number (LRN) by primary root length (PRL). Lateral root number (LRN) and primary root length (PRL) were previously derived from image analysis based - measurements from RR2D. (MO_001)
Leaf
The concentration of Rubidium in leaves
LfRb
Rubidium content
Leaf Rubidium content
Oleic seed content NIRS - Measurement
NIRS assay. Pct total fatty acids
BBCH growth stage 81;CETIUM growth stage G5
Plant
10% of the seeds have reached maturity, seeds are brown and hard Corresponds to growth stage G5 (CETIOM) or 81 (BBCH scale)
Beginning of pod maturity time (BBCH 81)
Development stage
Moisture content
SdMoist
Seed
Seed moisture content
Seed moisture content.
Seed protein content
Seed
The concentration of proteins in seeds.
Protein content
SdProt
Measurement of stem width approx 2 cm from base from randomly selected plants,using SigmaScan from digital image.
Stem witdh - Measurement
NIRS assay Quantity per Seed Dry Weight
GLS seed content NIRS - Measurement
Six plants were sampled from each plot, each plant was assessed for appearance of powdery mildew, if powdery mildew was present, it is recorded as 1
Powdery mildew presence - Estimation
LLS resistance - Estimation
10 plants from each plot were assessed on severity of light leaf spot (LLS) on the leaf. Assessment is based on a 0-7 score: 0=No disease observable, 1=Trace of disease, 2=Diseased leaves with non or a few small lesions, 3= Plants with scattered lesions, 4=Diseased leaved with a few large lesions of many small leasions, area quite heavily infected, 5= Half green/half diseased, leaf curling and distortion, 6= Evidence of stunting, 7=Plants heavily stunted or dead. This is based on the leaves of the whole plant. For example, if a plant had only scattered lesions on different leaves, it will score 3; if the same plant was heavily stunted, it will score 7.
Surface is determined by the experimental design
Plot Size – Computation
Seed
Seed Manganese content
Manganese content
The concentration of Manganese in seeds
SdMn
Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa
Phoma stem canker severity
Stem
Phoma stem cancer severity
mg
Seed moisture content humidifier - Measurement
Humidimeter (sample of seeds)
Pod and stem
The concentration of components with secondary alcohol functional groups from pod and stem material
SdAlcII
secondary Alkohol functional group content
Pod and stem secondary Alkohol functional group content
Plant
Phoma damage
Withering disease
Withering disease caused by the agent Leptosphaeria maculans (Phoma).
Phoma damage
PhomaDmg
Additional flowering stages according to the BBCH scale (62-65)
flowering stage;time to BBCH growth stage 62-65;Anthesis
Plant
Flowering time (BBCH 62-65)
Development stage
Seed
Avenasterol proportion of Phytosterols
The percentage of Avenasterol in the total of Phytosterols in seeds
SdAvenasterol_Cp
Avenasterol
Seed Avenasterol content
Iron content
LfFe
Leaf Iron content
The concentration of Iron in leaves
Leaf
Seed
Brassicasterol
Brassicasterol content
The concentration of Brassicasterol in seeds
Seed Brassicasterol content
SdBrassicasterol
10% of the pods have reached their final size, flowering of secondary inflorescences. Corresponds to growth stage G1 (CETIOM) or 71 (BBCH scale)
BBCH growth stage 71;CETIUM growth stage G1
Plant
Beginning of pod development time (BBCH 71)
Development stage
Development stage
Additional stages for pod maturity according to the BBCH scale (82-99)
pod maturity stage;time to BBCH growth stage 82-99
Pod maturity time (BBCH 82-99)
Plant
Count the number of plants on linear sections defined in each sowing line at the exception of the border lines
Plant stand - Counting
Plant
Oil yield
Oil yield
Oil yield
OY
SdErucic
Seed erucic acid content
Seed
Erucid acid content
The concentration of erucic acid in seeds.
NIRS assay. Pct total fatty acids
Palmitic seed content NIRS - Measurement
Count number of fertile branches. Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing.
Fertile Branches - Counting
The concentration of oil in seeds.
Seed
Seed oil content
SdOil
Oil content
Phoma stem cancer severity - Estimation
Six plants per plot were assessed for severity of phoma stem canker. For assessing the stem canker severity, the stem were cut the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged))
2020-10-19T00:00:02
Seeds were sieved using mesh with a diameter (Ø) of 1.4, 1.7, 2.0 and 2.36 mm. Seeds retained were recorded in the corresponding diameter category and treated as if this was their true diameter.
Seed sieving - Estimation
The concentration of Calcium in leaves
LfCa
Leaf
Calcium content
Leaf Calcium content
LLS severity - Estimation
10 plants were sampled from each plot, each plant was assessed for light leaf spot on all pods and the % diseased area estimated.
Seed
Seed linolenic acid content
The concentration of linolenic acid in seeds.
StLinolenic
Linolenic acid content
Plant
Phoma leaf spots
The number of plants that showed presence of phoma leaf spots from a subsample of a plot
Number of plants with phoma leaf spots
Aluminium content
The concentration of Aluminium in leaves
Leaf Aluminium content
Leaf
LfAl
Measure weight of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser.
Seed weight - Measurement
Rubidium content
SdRb
Seed Rubidium content
Seed
The concentration of Rubidium in seeds
Lateral root density
The mean density of lateral roots along the primary root
Lateral root
density
LRD
kg
Pod shattering - Estimation
Visual notations (scale 0 - 9)
Plant
Development stage
Additional stages for pod development according to the BBCH scale (72-79)
Pod development time (BBCH 72-79)
pod development stage;time to BBCH growth stages 72-79
Sda-toc
alpha-tocopherol content
seed
Seed alpha- tocopherol content
Alpha- tocopherol content in seeds
Caesium content
The concentration of Caesium in leaves
LfCs
Leaf Caesium content
Leaf
plant/m2
plants/plot
Six plants per plot were assessed for severity of phoma stem canker on the upper stem. For assessing the upper stem lesion severity, the stem were cut at the upper stem area, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged))
Phoma stem cancer severity - Estimation
Verticilium wilt presence - Estimation
Scoring of presence or absence of verticilium wilt symptoms across whole plant.
0-5 percentage bracket scale
The concentration of Avenasterol in seeds
Avenasterol content
SdAvenasterol
Seed Avenasterol content
Avenasterol
Seed
Additionnal pod development stages (BBCH 72-79) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
Cycloartenol proportion of Phytosterols
SdCycloartenol_Cp
The percentage of Cycloartenol in the total of Phytosterols in seeds
Seed
Cycloartenol
Seed Cycloartenol content
Leaf
Incidence with leaf miner
Leaf miner incidence
The percentage of plants that showed presence of leaf miner from a subsample of a plot
SdCycloartenol
The concentration of Cycloartenol in seeds
Cycloartenol content
Cycloartenol
Seed Cycloartenol content
Seed
q/ha
Plot measure validity – Estimation
Annotations on plot measure validity. Possible values for a VALID measure : “valid” or empty and for INVALID measures (separated by comma) : “prob_BIOTIC” (problem from biotic attacks), “prob_LODGING” (problem with lodging), “prob_CLIMATIC” (problem with climatic incidents), “not valid”
Plant stand at emergence
Plant emergence
Seed
Percentage of seeds that emerge.
PltSdEm
Seed Potassium content
Seed
The concentration of Potassium in seeds
Potassium content
SdK
plants/6 plants
LLS severity on leaf
Leaf
Light Leaf spot severity on leafs
Light leaf spot resistance
The concentration of Arsenic in seeds
Seed
Arsenic content
Seed Arsenic content
SdAs
The concentration of components with fatty acids as functional groups from pod and stem material
Fatty acids functional group content
Pod and stem Fatty acids functional group content
SdFA
Pod and stem
The sum of primary root length (PRL) and lateral root length (LRL) was used to obtain total root length (TRL). Root lengths were previously derived from image analysis based - measurements from RR2D. (MO_001)
Image analysis - Computation
Seed
SdCa
Seed Calcium content
The concentration of Calcium in seeds
Calcium content
number
Number of seeds in ten typical pods
seed
Seed number
Plant
The percentage of plants that showed presence of leaf miner from a subsample of a plot
Verticillium wilt incidence
Verticilium wilt presence
First inter-node (BBCH 31) date – Estimation
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
The whole plot was assessed and the number of plants which showed Sclreotinia stem rot presence on stem or side branch was recorded.
Sclerotinia incidence (diseased) - Counting
Measurement of pod beak length (cm) is taken as the average from ten typical pods measured using SigmaScan from digital image of ten pods per plant.
Pod beak length - Measurement
Seed
Protein yield per ha
Seed protein yield
Protein yield
PrY
length
Lateral root length
Lateral root
LRL
Length of the lateral root
Foot length
foot
Length of lower stem from ground level to last formed fertile branch
length
SdCu
The concentration of Copper in seeds
Seed Copper content
Copper content
Seed
The concentration of all compounds with branched alcyl chains from pod and stem material
branched Alcyl-chain content
Pod and stem branched Alcyl-chain content
Pod and stem
SdBranched
Seed
Percentage of impurities in a sample of seeds determined by weighing.
Impurities content
Seed impurity content
SdImpur
pod
number
Pod number
Number of pods on terminal raceme
CETIOM (2005), Guide Expérimentateur Colza, Fiche A1: 'Bien repérer les stades du colza'. Echelle BBCH.
First flowers (BBCH 60) date – Estimation
Upper stem
Severity assessment of phoma stem canker upper stem lesion score. It is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa
Phoma stem cancer severity
Phoma stem canker upper stem lesion score
Image analysis - Computation
The average of length of lateral roots was derived from dividing lateral root length (LRL) by lateral root number (LRN). Lateral root lengths and numbers were previously derived from image analysis based - measurements from RR2D. (MO_001)
Yield
Weight (kg) of raw materials harvested by plot
Biomass
Biomass yield
Image analysis - Counting
RootReader2D (RR2D, Clark et al., 2013) was used to analyse images. First, a ‘batch process’ was carried out which automatically ‘thresholds’, ‘skeletonises’ and ‘builds segments’ of all images in bulk. Then, the the root system of individual images was measured by placing a marker at the ase and tip of the primary root. From these markers, RR2D automatically calculates primary root length (PRL), lateral root length(LRL) of all laterals and lateral root number (LRN).
Seed impurity content – Computation
Differential weighing. A sample of around 200 grammes ofdrymature seeds is weighted before and after cleaning using a column in order to determine the gross weight (GWg) and the net weight (NWg) respectively
SdAld
Pod and stem Aldehyde functional group content
The concentration of components with aldehyde functional groups from pod and stem material
Aldehyde functional group content
Pod and stem
Sodium content
LfNa
Leaf Sodium content
The concentration of Sodium in leaves
Leaf
Measurement of mean length of pedicel (cm), using SigmaScan from digital image of 10 typical pods from terminal raceme or primary raceme if terminal was damaged
Pedicel length - Measurement
method_of
Plant lodging incidence - Estimation
Visual notation (scale 0 - 9) is attributed to the plants for each plot, taking into account the surface and the intensity of lodging
Seed Sigmasterol content
Stigmasterol content
Seed
SdStigmasterol
The concentration of Stigmasterol in seeds
Stigmasterol
SdSe
Seed Selenium content
Selenium content
Seed
The concentration of Selenium in seeds
Plant height - Measurement
Measure length of plant from ground level to tip of terminal raceme (cm). Plants are randomly selected from the centre of a well established plots avoiding obvious volunteers. All plant are harvested in the field and returned to the lab for processing.
mm
Nickel content
Leaf
The concentration of Nickel in leaves
Leaf Nickel content
LfNi
0-9 Lodging Incidence scale
stem
Stem width
Stem width measured at the midpoint of a 5cm sample collected from the base of a hand harvested plant
width
Plant stand at emergence – Computation
EMERG = (Number of plants obtained / Number of seeds sown) x 100
number
Lateral root number
Lateral root
Number of lateral roots
LRN
μmol/g
Seed moisture content – Computation
Differential weighing. A sample of around 5 grammes of cleaned dry mature seeds is weighted before and after drying at 103°C for 15 hours in order to determine the fresh weight (FWg) and the dry weight (DWg) respectively
PRL
Primary root lengh
Primary root
Length of the primary root
length
Crop problems annotations - Estimation
Visual observations
width
pod
Pod width
Pod width of pods from terminal raceme
Phoma stem cancer severity - Estimation
Six plants per plot were assessed for severity of phoma stem canker on the base of the stem. For assessing the base stem canker severity, the stem were cut at the stem base, the cross-section was scored on a 0-7 scale ( 0= no symptoms observable, 1 = ≤ 5% girdling,2= 2-25% girdling, 3=26-50% girdling, 4=51-75% girdling, 5= 75-100% girdling, 6=100% girdling ( stem weak/firm), 7 = 100% girdling (stem dead/lodged))
Count number of seeds in ten typical pods taken from main raceme (or primary branch if main raceme damaged) using MARVIN seed analyser.
Seed Number - Counting
Stem base
Phoma stem cancer severity
Phoma stem canker base stem score
Severity assessment of phoma stem canker base stem on a 0-7 scale. Stem canker is a fungal infection of ascomycete species Leptosphaeria maculans and L. biglobosa
Sclerotinia incidence (diseased) - Counting
Six plants were sampled and each plant was assessed for the presence/absence of Sclerotinia stem rot, number of plants with Sclerotinia was recorded (for the second replicate).
seed
10 plants from each plot were sampled and the leaf miner incidence was assessed. Plants were assessed for the presence or absence of leaf miner. The incidence of leaf miner was calculated as % of plants out of the ten sampled plants that were found to have leaf miner.
Leaf miner incidence (diseased) - Counting
scale_of
Number of fertile branches
Number of fertile branches
number
fertile branch
Light leaf spot severity
Light leaf spot severity on stems, judged by the percentage of area covered by LLS.
LLS severity on stem (percentage of stem area covered by LLS)
Stem
The concentration of Chromium in seeds
Seed
SdCr
Seed Chromium content
Chromium content
boolean
Visual notations (scale 0 - 9)
regularity stand - Estimation
NIRS assay. Pct total fatty acids
Stearic seed content NIRS - Measurement
Oil yield calulated as a product of seed yield (SY) and oil content in seeds (O)
Oil yield - Computation
Nickel content
Seed
The concentration of Nickel in seeds
Seed Nickel content
SdNi
0-3 disease stage scale
Visual observations
Climatic incidents annotions - Estimation
0-7 disease stage scale
0-9 Regularity scale
Score the damage caused by hail.
HailDmg - Estimation
Phoma damage – G2 score – Computation
Evaluation of the Phoma lingam infection with the G2 score.
The evaluation occur in the stage G4 of the crop on the collar and on the elongation. Dig out the plant, Realize a cross section at the collar with a pruning, Classify the collars with the rating scale : 1= healthy plant ; 2=0-25% of necrotized surface, 3=25-50% of necrotized surface, 4=50-75% of necrotized surface, 5=75-100% of necrotized surface, 6=plant broke during the evaluation.
variable_of